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Using a 7000smz-2 Vibratome to produce Human Precision Cut Liver Slices
What are Precision Cut Liver Slices? Precision-cut liver slices (PCLS) are thin, uniform sections of liver tissue used in research to...

Ian Davies
Feb 12, 20242 min read
12 views


50µm Mouse Brain Sections Cut with 7000smz Vibrating Microtome for Immunohistochemistry and Imaging
Abstract (from Early alterations in the MCH system link aberrant neuronal activity and sleep disturbances in a mouse model of Alzheimer’s...

Ian Davies
May 18, 20232 min read
20 views


Pancreatic ductal adenocarcinoma tumor slices (300–350µm) using 7000smz-2 vibrotome
Abstract (from Protocol to culture and image pancreatic ductal adenocarcinoma tumor slices to study T cell migration, Rodrı´guez-Merced...

Ian Davies
Feb 28, 20232 min read
9 views


300-350µm Horizontal Hippocampal Slices cut with 7000smz Vibratome
Abstract (from Importin a5 Regulates Anxiety through MeCP2 and Sphingosine Kinase 1, Panayotis et al, 2018) Importins mediate transport...

Ian Davies
Jan 27, 20231 min read
6 views


Immunofluorescence Staining in 100µm - 200µm Mouse Tumour Slices
Abstract (from Endothelial cell death after ionizing radiation does not impair vascular structure in mouse tumor models, Kaeppler et al,...

Ian Davies
Aug 3, 20222 min read
15 views


Investigation of the role of axonal voltage-gated potassium channels in Direct Current Stimulation
Abstract (from The role of axonal voltage-gated potassium channels in tDCS, Vasu and Kaphzan, 2022) Background: Transcranial direct...

Ian Davies
Jun 21, 20222 min read
23 views


80µm fixed brain slices (5100mz); Calcium Imaging & Patch study in 300µm brain slices (7000smz-2)
Abstract (from Designing AAV Vectors for Monitoring the Subtle Calcium Fluctuations of Inferior Olive Network in vivo, Dorgans et al,...

Ian Davies
May 5, 20222 min read
19 views


Human Left Ventricular 400µm Slices for Optical Imaging cut with 7000smz-2
Abstract (from Acetylcholine Reduces IKr and Prolongs Action Potentials in Human Ventricular Cardiomyocytes, Koncz et al, 2022) Vagal...

Ian Davies
Feb 3, 20222 min read
21 views


Cardiomyocyte Isolation from 300µm rabbit left atrial & left ventricular slices
Abstract (from Consecutive-Day Ventricular and Atrial Cardiomyocyte Isolations from the Same Heart: Shifting the Cost–Benefit Balance of...

Ian Davies
Jan 18, 20223 min read
16 views


Mice Brain Slices (40-100µm) cut with 5100mz Vibrotome for Immunofluorescence
Abstract (from Functional consequences of postnatal interventions in a mouse model of Fragile X syndrome, Rais et al, 2021) Background...

Ian Davies
Dec 9, 20212 min read
53 views


70µm Mouse Brain Sections for Immunohistochemistry cut with 5100mz Vibrotome
Abstract (from PUPIL enables mapping and stamping of transient electrical connectivity in developing nervous systems, Xie et al, 2021)...

Ian Davies
Nov 26, 20211 min read
16 views


Slicing and Culturing Pig Hearts under Physiological Conditions
Abstract (from Slicing and Culturing Pig Hearts under Physiological Conditions, OU et al., 2020) Many novel drugs fail in clinical...

Ian Davies
Oct 12, 20212 min read
22 views


350µm - 450µm Sagittal Brain Sections for LFP and Whole Cell Patch Recordings, & Immunofluorescence
Abstract (from Multimodal electrophysiological analyses reveal that reduced synaptic excitatory neurotransmission underlies seizures in a...

Ian Davies
Sep 29, 20214 min read
17 views


Patch & Current Clamp in 300µm Medial Prefrontal Cortex Slices cut with 7000smz-2
Abstract (from Excitatory synapses and gap junctions cooperate to improve Pv neuronal burst firing and cortical social cognition in...

Ian Davies
Sep 1, 20212 min read
19 views


Immunohistochemistry of Microglial Cells in the Rat Hippocampus sectioned with 7000smz
Abstract (from Effects of psychogenic stress on some peripheral and central inflammatory markers in rats with the different level of...

Ian Davies
Aug 4, 20212 min read
7 views


Whole-Cell Voltage-Clamp in 350µm Mouse Coronal Brain Slices
Abstract (from Npas4 regulates IQSEC3 expression in hippocampal somatostatin interneurons to mediate anxiety-like behavior, Kim et al,...

Ian Davies
Jul 29, 20214 min read
7 views


Electrophysiology in 300µm mouse DMH Slices in Study Investigating Regulation of AGRP Neurons
Abstract (from Food cue regulation of AGRP hunger neurons guides learning, Berrios et al, 2021) Agouti-related peptide (AGRP)-expressing...

Ian Davies
Jul 22, 20212 min read
16 views


Extracellular Recording from Coronal, 400µm Hippocampal Slices to Investigate Synaptic Transmission
Abstract (from miRNA-132/212 Gene-Deletion Aggravates the Effect of Oxygen-Glucose Deprivation on Synaptic Functions in the Female Mouse...

Ian Davies
Jul 14, 20212 min read
14 views


500µm Slices of Live Right Ventricular Outflow Tract cut for SHGI-based fibrosis quantification
Abstract (from Quantitative collagen assessment in right ventricular myectomies from patients with tetralogy of Fallot, Wülfers et al,...

Ian Davies
Jun 23, 20212 min read
17 views


250µm Coronal Slices of Somatosensory ‘barrel’ Cortex Prepared using 7000smz
Abstract (from Transient developmental imbalance of cortical interneuron subtypes presages long-term changes in behavior, Magno et al,...

Ian Davies
Jun 22, 20212 min read
6 views
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