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7610A Temperature Controller - Cooling

  • Utilitizes the standard inner stainless steel tissue bath

  • Display Resolution: 0.1° C

  • Temperature Accuracy: +/- 1° C

  • Temperature Range: +8-0° C**

  • Voltage Requirements: 230V 50Hz or 115V 60Hz

  • Power Rating: 60W

  • Inlet Fuse Rating: 2A

 

** Actual temperatures achievable will be dependent upon the solutions used and local temperature conditions. For optimal cooling; inlet water temperature should be below 25° C and flow rate should be greater than 400ml/min

Details

Traditionally once removed from the animal, tissue is immediately cooled to close to +4° C to lower the oxygen demand and prevent anoxia. This has the added advantage with delicate tissues of increasing the stiffness of the tissue allowing for a more robust slice. This can be done with passive cooling by applying ice to the outer icebath (included as standard) or use of the 7610A Tissue Cooler Bath.

 

The advantage of the 7610A is constant tight control of the bath temperature over long periods without the requirement to refresh the ice or disturb the tissue sample.

 

The control unit supplies power to the 'Peltier' thermoelectric elements in the base of the tissue bath. These act as energy transfer units so that heat is drawn off, through the heat exchanger and removed by tap water flow which runs to waste. The unit uses P.I.D. (proportional integral derivative) temperature control to take the bath temperature to within 0.5° C at the point of measurement. The temperature feedback thermistor is located in the bath floor and software calculates an offset automatically.

Further Information

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Stuart Greenhill, PhD,

Aston University

Our laboratory uses both animal and human tissue in acute and organotypic culture brain slice experiments. We have two 7000smz-2 vibrotomes in daily use which form the backbone of our slice preparations – the reliability and repeatability provided by the slicer’s Z-axis stability and calibration mechanism means that our preparations get off to the best possible start. The 7000smz-2 produces the highest quality brain slices of any vibrotome I have used, allowing us to generate in vivo-like oscillations in slice preparations. On top of this the service and support from Campden has been second to none.

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